Movie S12 - High-density 3D imaging of intracellular and plasma membranes in a telophase cell from optical membrane Watch Video

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⏲ Duration: 83 sec
✓ Published: 02-Mar-2016
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Orthoslices of the telophase LLCPK1 cell from Supplementary Figure 15 showing intracellular membranes labeled with BODIPY TR methyl ester and plasma membrane labeled with wheat germ agglutinin–Alexafluor 555. Similar to the interphase COS-7 cell from Supplementary Video 5, internalized plasma membrane lined vesicles are also visible in this dividing LLCPK1 cell.nnBackground: In these movies, scientists at the Howard Hughes Medical Institute's Janelia Research Campus combine ultrathin light she

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Volume rendering of the telophase LLCPK1 cell from Supplementary Figure 15 showing intracellular membranes labeled with BODIPY TR methyl ester and plasma membrane labeled with wheat germ agglutinin–Alexafluor 555. Also shown is a diffraction-limited, deconvolved dithered lattice light sheet image of mCherry-Histone H2B. Thin retraction fibers that anchor the cell to the substrate are clearly visible. Volume rendering encompasses a 50 × 26.8 × 19.7 μm field of view.nnBackground: In these mov
⏲ 45 sec ✓ 02-Mar-2016
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